We demonstrate that SpyC has broad utility as a protein-fusion tag partner in a eukaryotic expression/secretion context, retaining its functionality and permitting the direct, selective capture and immobilization of soluble antigen fusions using solid phase media coated with a synthetic modified SpyT peptide reagent. In addition, we show that the expressed SpyC-antigen format is highly compatible with downstream antibody phage display selection and screening procedures, requiring minimal post-expression handling with no sample modifications. To illustrate the potential of the approach, we have isolated several fully human germline scFvs that selectively recognize therapeutically relevant native cell surface tumor antigens in various in vitro cell-based assay contexts.

A magnetic bottle is two magnetic mirrors placed close together. For example, two parallel coils separated by a small distance, carrying the same current in the same direction will produce a magnetic bottle between them. Unlike the full mirror machine which typically had many large rings of current surrounding the middle of the magnetic field, the bottle typically has just two rings of current.