The Mac
@TheMac
05 May, 06:28
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Jodie Jennings
@Jodie
05 May, 06:35
In response The Mac to his Publication
Your info is fascinating. Could you make one post and explain it further?
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The Mac
@TheMac
05 May, 06:44
In response Jodie Jennings to her Publication
🙂
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The Mac
@TheMac
05 May, 06:44
In response The Mac to his Publication
Magnetogenetics refers to a biological technique that involves the use of magnetic fields to remotely control cell activity.
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The Mac
@TheMac
05 May, 06:45
In response The Mac to his Publication
In most cases, magnetic stimulation is transformed into either force (magneto-mechanical genetics) or heat (magneto-thermal genetics), which depends on the applied magnetic field.
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The Mac
@TheMac
05 May, 06:46
In response The Mac to his Publication
Therefore, cells are usually genetically modified to express ion channels that are either mechanically or thermally gated.
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The Mac
@TheMac
05 May, 06:47
In response The Mac to his Publication
As such, magnetogenetics is a cellular modulation method that uses a combination of techniques from magnetism and genetics to control activities of individual cells in living tissue – even within freely moving animals.
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The Mac
@TheMac
05 May, 06:47
In response The Mac to his Publication
This technique is comparable to optogenetics, which is the manipulation of cell behavior using light. In magnetogenetics, magnetic stimulation is used instead of light, a characteristic that allows for a less invasive, less toxic, and wireless modulation of cell activity.
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The Mac
@TheMac
05 May, 06:48
In response The Mac to his Publication
Iron oxide (Fe₃O₄) nanoparticles (IONPs) have received much attention for their utility in biomedical applications such as magnetic resonance imaging, drug delivery and hyperthermia.
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The Mac
@TheMac
05 May, 06:49
In response The Mac to his Publication
Recent studies reported that IONPs induced cytotoxicity in mammalian cells.
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The Mac
@TheMac
05 May, 06:50
In response The Mac to his Publication
However, little is known about the genotoxicity of IONPs following exposure to human cells. In this study, we investigated the cytotoxicity, oxidative stress and genotoxicity of IONPs in two human cell lines; skin epithelial A431 and lung epithelial A549. Prepared IONPs were polygonal in shape with a smooth surface and had an average diameter of 25 nm.
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IONPs (25-100 μg/ml) induced dose-dependent cytotoxicity in both types of cells, which was demonstrated by cell viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) and lactate dehydrogenase leakage assays. IONPs were also found to induce oxidative stress in a dose-dependent manner, evident by depletion of glutathione and induction of reactive oxygen species (ROS) and lipid peroxidation.
06:50 AM - May 05, 2021
In response The Mac to his Publication
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The Mac
@TheMac
05 May, 06:51
In response The Mac to his Publication
Comet assay revealed that level of DNA damage was higher with concentration of IONPs in both types of cells. Quantitative real-time PCR analysis showed that following the exposure of cells to IONPs, the expression levels of mRNA of caspase-3 and caspase-9 genes were higher.
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The Mac
@TheMac
05 May, 06:51
In response The Mac to his Publication
We also observed the higher activity of caspase-3 and caspase-9 enzymes in IONPs treated cells. Moreover, western blot analysis showed that protein expression level of cleaved caspase-3 was up-regulated by IONPs in both types of cells. Taken together, our data demonstrates that IONPs have potential to induce genotoxicity in A431 and A549 cells, which is likely to be mediated through ROS generation and oxidative stress. This study suggests that genotoxic effects of IONPs should be further investigated at in vivo level.
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