The Mac
@TheMac
14 February, 08:56
I just need to repost this.
Electroporation, or electropermeabilization, is a microbiology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs, or DNA to be introduced into the cell (also called electrotransfer).
Electroporation, or electropermeabilization, is a microbiology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs, or DNA to be introduced into the cell (also called electrotransfer).
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The Mac
@TheMac
14 February, 08:57
In response The Mac to his Publication
[2][3] In microbiology, the process of electroporation is often used to transform bacteria, yeast, or plant protoplasts by introducing new coding DNA.
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The Mac
@TheMac
14 February, 08:57
In response The Mac to his Publication
If bacteria and plasmids are mixed together, the plasmids can be transferred into the bacteria after electroporation, though depending on what is being transferred cell-penetrating peptides or CellSqueeze could also be used.
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The Mac
@TheMac
14 February, 08:58
In response The Mac to his Publication
Electroporation works by passing thousands of volts across a distance of one to two millimeters of suspended cells in an electroporation cuvette (1.0 – 1.5 kV, 250 – 750 V/cm).
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[contradictory] Afterwards, the cells have to be handled carefully until they have had a chance to divide, producing new cells that contain reproduced plasmids. This process is approximately ten times more effective than chemical transformation.[2][4]
08:58 PM - Feb 14, 2021
In response The Mac to his Publication
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The Mac
@TheMac
14 February, 09:01
In response The Mac to his Publication
"volts across a distance of one to two millimeters"
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The Mac
@TheMac
14 February, 09:04
In response The Mac to his Publication
A voltage-controlled oscillator (VCO) is an electronic oscillator whose output frequency is proportional to its input voltage. An oscillator produces a periodic AC signal, and in VCOs, the oscillation frequency is determined by voltage.
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