The Mac on AnonUp.com

14 February, 08:56

I just need to repost this.

Electroporation, or electropermeabilization, is a microbiology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs, or DNA to be introduced into the cell (also called electrotransfer).

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The Mac @TheMac

14 February, 08:57

In response The Mac to his Publication

[2][3] In microbiology, the process of electroporation is often used to transform bacteria, yeast, or plant protoplasts by introducing new coding DNA.

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The Mac @TheMac

14 February, 08:57

In response The Mac to his Publication

If bacteria and plasmids are mixed together, the plasmids can be transferred into the bacteria after electroporation, though depending on what is being transferred cell-penetrating peptides or CellSqueeze could also be used.

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The Mac @TheMac

Electroporation works by passing thousands of volts across a distance of one to two millimeters of suspended cells in an electroporation cuvette (1.0 – 1.5 kV, 250 – 750 V/cm).

08:58 PM - Feb 14, 2021

In response The Mac to his Publication

The Mac @TheMac

14 February, 08:58

In response The Mac to his Publication

[contradictory] Afterwards, the cells have to be handled carefully until they have had a chance to divide, producing new cells that contain reproduced plasmids. This process is approximately ten times more effective than chemical transformation.[2][4]