Hi Joe, my daughter is a veterinarian, and we were just discussing Lyme and Anaplasmosis last night, for a friend of ours situation. Our area of Oh has a lot of lyme disease the past few years! She does large animal, as well as small, in the largest Amish community in Oh. They have repeatedly given doxycycline successfully, which takes care of the lyme. Doxy is also used on humans for lyme. Don't know dosage for a pup, but I'm sure any vet could figure it out based on weight, with the pharm. instructions Good luck with the pup, and keep us in the loop