The pull-down assay is a technique developed to verify the physical interactions between proteins in vitro, which can be also used to confirm a predicted protein-protein interaction and identify novel interacting partners. Although this method can't reflect the real interaction under physiological conditions due to the uncertain spatially encounter in vivo, it is a powerful tool for studying protein-protein interactions in vitro. This method typically involves the affinity capture process using bait protein coupled supports and various wash and elution steps, which are tedious and time-consuming. https://www.cd-bioparticle...