The fundamental feature of Orthogonal Fab's design is its capacity to self-assemble from two distinct antibodies by altering the Fab interface. The area where the heavy and light chains interact is known as the Fab interface. The Fab interface is designed to create orthogonal heavy chain-light chain pairs using computational and rational design techniques. This guarantees that each light chain bonds more strongly to its cognate heavy chain than to the noncognate heavy chain. By using strategic engineering, the problem of light chain mispairing is lessened and the proper assembly of bispecific IgG is guaranteed. With its native-like IgG structure, Orthogonal Fab maintains its well-known stability and solubility.

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