Here we use multilayer gate technology to create a device based on two distinct phases in adjustable regions of MATBG. We electrostatically define the superconducting and insulating regions of a Josephson junction and observe tunable d.c. and a.c. Josephson effects15,16. The ability to tune the superconducting state within a single material circumvents interface and fabrication challenges, which are common in multimaterial nanostructures. This work is an initial step towards devices where gate-defined correlated states are connected in single-crystal nanostructures. We envision applications in superconducting electronics17,18 and quantum information technology19,20.