The Mac @TheMac
04 May, 07:05
NEW WHIRLED ORDER

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The Mac @TheMac
04 May, 07:13
In response The Mac to his Publication

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The Mac @TheMac
04 May, 07:14
In response The Mac to his Publication

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The Mac @TheMac
04 May, 07:16
In response The Mac to his Publication

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The Mac @TheMac
04 May, 07:16
In response The Mac to his Publication

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The Mac @TheMac
04 May, 07:17
In response The Mac to his Publication

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The Mac @TheMac
04 May, 07:34
In response The Mac to his Publication

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The Mac @TheMac
04 May, 07:37
In response The Mac to his Publication
entity (plural entities)

That which has a distinct existence as an individual unit. Often used for organisations which have no physical form.

The existence of something considered apart from its properties.

(databases) Anything about which information or data can be stored in a database; in particular, an organised array or set of individual elements or parts.

The state or quality of being or existence.

The group successfully maintains its tribal entity.

A spirit, ghost, or the like.

(science fiction) An alien lifeform that has no corporeal body.

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The Mac @TheMac
04 May, 07:39
In response The Mac to his Publication
Ghost

mid 19th century: from German Poltergeist, from poltern ‘create a disturbance’ + Geist ‘ghost’.

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The Mac @TheMac
04 May, 07:42
In response The Mac to his Publication
The word poltergeist comes from the German language words poltern ("to make sound" and "to rumble") and Geist ("ghost" and "spirit"), and the term itself translates as "noisy ghost", "rumble-ghost" or a "loud spirit". A synonym coined by René Sudre is thorybism, from Greek θορυβείν ("to make noise or uproar; throw into confusion").

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The Mac @TheMac
04 May, 07:47
In response The Mac to his Publication

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The Mac @TheMac
04 May, 07:57
In response The Mac to his Publication

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The Mac @TheMac
04 May, 07:59
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:00
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:01
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:03
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:04
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:09
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:09
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:09
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:09
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:10
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:10
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:10
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:10
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:11
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:11
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:11
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:12
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:12
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:13
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:13
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:14
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:14
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:15
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:16
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:16
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:19
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:20
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:20
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:20
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:21
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:22
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:25
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:26
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:26
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:27
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:28
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:28
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:28
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:28
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:29
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:35
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:36
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:37
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:40
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:42
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:45
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:51
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:52
In response The Mac to his Publication

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The Mac @TheMac
04 May, 08:56
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:06
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:06
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:07
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:07
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:08
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:09
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:09
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:10
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:11
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:11
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:12
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:16
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:17
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:18
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:20
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:22
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:22
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:22
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:28
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:29
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:29
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:30
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:31
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:32
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:33
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:35
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:38
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:42
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:42
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:43
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:43
In response The Mac to his Publication

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04 May, 09:45
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Only people mentioned by TheMac in this post can reply
The Mac @TheMac
04 May, 09:45
In response The Mac to his Publication

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The Mac @TheMac
04 May, 09:49
In response The Mac to his Publication
Principle of FRET and BRET in protein interaction sensors used in this work. Note that the FRET sensor (A) requires an external light source for the excitation of the donor fluorophore. Donor and acceptor are fused to a subunit of a trimeric G protein, respectively. Activation of the G protein by an agonist-bound receptor will lead to movement of the subunits relative to each other, thereby changing the fluorophores' distance from and/or orientation towards each other. The donor in BRET (B) is a luciferase fused to the receptor, which emits light of a distinct wavelength spectrum in the presence of its substrate. When the β-arr, which is fused to the acceptor fluorophore, binds to the activated receptor, the two fluorophores will be in close enough proximity for resonance energy transfer to occur. In both applications, donor and acceptor emissions are recorded separately and the ratio of both serves as a quantitative readout.

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