Lipids are hydrophobic or amphipathic small molecules which include fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides and phospholipids. The important roles of lipids in biological physiology are evident not only in energy storage and structural components of cellular membranes, but also in signal transduction, membrane trafficking and morphogenesis. lipid content analysis is the synthesis and degradation of lipids in cells, consisting of the breakdown or storage of fats for energy and the synthesis of structural and functional lipids like those involved in the construction of cell membranes. https://metabolomics.creat...

Inositol phosphates contain multiple phosphorylated derivatives and individual PIPs can contain different fatty acid chains, resulting in structural complexity. Creative Proteomics has built a dedicated analytical platform to provide you with qualitative and quantitative analysis of inositol phosphates. Based on ion chromatography separation techniques, we are able to accurately determine the total amount of phosphorylated derivatives. Based on LC/MS technology, we are able to quantify inositol phosphate pathway containing specific fatty acid chain structures. https://metabolomics.creat...

Starch is a polymeric carbohydrate consisting of a large number of glucose units connected by glycosidic bonds. Similarly, sucrose is a kind of carbohydrate and composed of two monosaccharides: glucose and fructose. Basically, the starch and sucrose metabolism begin with D-fructose that interacts with a D-glucose in a reversible reaction through a maltodextrin glucosidase. By multiple procedures, the metabolism of starch and sucrose produce several important intermediates like glucose-6-phosphate and fructose-6-phosphate, which interact with other metabolic pathways including glycolysis and gluconeogenesis. Creative Proteomics has advanced mass spectrometry instrumentation and a team of bioinformatic analysts to provide starch analysis method. https://metabolomics.creat...

Apoptosis plays an important role in the immune system, which is closely related to intracellular homeostasis, T cell aggregation, death of reactive lymphocytes and cytotoxic response of target cells. At present, it has been found that eukaryotic cell apoptosis mainly has the following pathways: 1. Cell receptor-mediated external apoptotic pathways; 2. Internal mitochondrial pathway; 3. B-granase mediated apoptosis pathway; 4. Endoplasmic reticulum stress pathway mediates apoptosis. The detection of cytokines related to apoptosis helps to study apoptosis and the physiological or pathological processes involved. Creative Proteomics can simultaneously quantitatively detect apoptosis and proinflammatory cytokines through a variety of technologies, and provide higher sensitivity and a wider detection range. https://cytokine.creative-...

Inflammatory cytokines can regulate the response of pro-inflammatory cytokine production, mainly including IL-4, IL-5, IL-10, IL-13, transforming growth factor (TGF), epiodermal growth factor and so on. Among them, IL-4 can inhibit the production of other pro-inflammatory cytokines such as IL-1 and IL-6, as well as the production of lymphocytes and macrophages. IL-10 can inhibit the production of activated monocytes, macrophages, granulocytes and T cells. And IL-13 can inhibit the production of pro-inflammatory transmitters and down-regulate the function of monocytes with cytotoxicity. TGF-β can inhibit the inflammatory response of intestine. And EGF can stimulate tissue repair and protect the mucosa of gastrointestinal tract. Creative Proteomics can provide cytokine detection platform for brain inflammation cytokine research. https://cytokine.creative-...

Serum and plasma samples provide a holistic view of systemic responses. These liquid components of blood circulate throughout the entire body, offering a comprehensive representation of the intricate cytokine networks at play. This approach ensures that the analysis captures the full spectrum of cellular communication, enabling a more nuanced understanding of physiological processes. Creative Proteomics can provide you with a one-stop serum cytokine analysis for cytokine and protein detection and quantification. https://cytokine.creative-...

Lipid modifications include Glycosyl-phosphatidylinositol anchor (GPI). At the C-terminus of proteins, it happens in the endoplasmic reticulum that the post-translational linkage between glycosylphosphatidylinositol and the modified proteins are translocated to the membrane, which are remain anchored to the outer leaflet of the cell membrane. Methods for detecting protein lipidation have been exploited to predict GPI-anchored proteins, N-myristoylated, S-isoprenylated or S-palmitoylated proteins, and so on. The traditional approaches are based on the incorporation of radioactive precursors and subsequent detection of the proteins by fluorography. Mass spectrometry (MS) methods are universally applied, which are for the mapping of post-translational modifications. This technology submits crucial advantages. Bio-orthogonal probes, reactions compatible with biomolecules that allow their study in their native settings. https://www.creative-prote...

Our protein C-terminal sequencing technology service can provide C-terminal sequencing analysis methods for biopharmaceutical proteins or other biological reagents such as MALDI-ISD for Top-down protein C-terminal sequencing and carboxypeptidase hydrolysis. Creative Proteomics is a reliable biopharmaceutical partner with professional proteomics research resources. We use first-class experimental platform and mature technical methods to provide you with high-quality one-stop service. https://www.creative-prote...

Circular dichroism (CD) spectroscopy is a fast, simple, and accurate method for studying protein structures in dilute solutions. The structural analysis was performed using the circular dichroism of proteins and the difference in absorption of left and right circularly polarized light by asymmetric molecules. As a reliable partner of biopharmaceutical companies, Creative Proteomics can provide customers with far UV circular dichroism spectroscopy analysis services based on GLP / GMP. The detectable molecules range from small chiral drugs and transition metal complexes to biological macromolecular structures such as proteins, glycoproteins and nucleic acids. https://www.creative-prote...

LC-MS/MS platform enable simultaneous relative and absolute quantification of multiple metabolites in a biological system. The results are widely used for biomarker discovery by investigating the relative changes in metabolite concentrations. The resulting data have been widely used to for biomarker discovery. By providing the exact molecular weights and retention time, LC-MS/MS techniques serves as a powerful analytical tool for identification and quantification of small molecules (metabolites). Creative Proteomics has established sensitive, reliable, and accurate HPLC-MS/MS method for quantification of metabolites in pentose phosphate pathway metabolites. https://www.creative-prote...

Lignin is one of the most abundant aromatic biopolymer in nature and accounts for nearly 30% of the plants. Its deposition in the cell wall plays vital roles for plant development, such as providing rigidity and strength to the cell wall to give structural support for the plant cell wall. Lignin exhibits hydrophobicity in favor of the transport of water and solutes in the vascular system. It also protects the cell against pathogens. As one of the leading companies in the omics field with over years of experience in omics study, Creative Proteomics provides glycomics analysis service customized to your needs. https://www.creative-prote...

Glycolysis pathway is a common metabolic pathway in both prokaryotic and eukaryotic cells. It takes place in the cytosol in eukaryotic cells. By converting glucose into two molecule of the three‐carbon compound pyruvate and generating two ATPs, glycolysis pathway generates the ATP needed for energy‐requiring processes and the pyruvate needed for the complete oxidation of glucose to carbon dioxide and water. With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective glycolysis pathway metabolites targeted metabolomics services. https://www.creative-prote...

Creative Proteomics offers the Luminex Human Inflammation 20-plex/24-plex/25-plex/27-plex/37-plex/48-plex Panel for quantifying human Inflammation factors in serum, plasma, tissue culture supernatants and cultured cells to meet your diverse needs. Base on Luminex xMAP technology, we can achieve ideal speed and sensitivity for simultaneous quantification of multiple analytes, significantly increasing productivity and saving valuable sample volumes. https://cytokine.creative-...

Cytokine arrays are capable of measuring the levels of many proteins simultaneously. Compared to single-target ELISA, immunoprecipitation, or protein blotting assays, cytokine array analysis offers greater efficiency, speed, and range of detection. Creative Proteomics has developed a variety of cytokine arrays that enable the simultaneous detection and accurate quantification of multiple secreted proteins (cytokines, chemokines, growth factors, etc.) and biomarkers, providing a solution for the comprehensive study of secreted protein factors in biological models, diseases, and organismal systems. https://cytokine.creative-...

Luminex multiplex detection is driven by xMAP technology, which can simultaneously detect and quantify more than 500 protein molecules, including cytokines, hormones, autoantibodies, tumor markers, etc. Creative Proteomics provides Luminex multiplex assay platform to help you accelerate the progress of the project, suitable for basic and clinical medical research. https://cytokine.creative-...

Targeted metabolomics based on mass spectrometry can quantitatively analyze chlorophyll in a more sensitive and more accurate manner. With decades of experience in mass spectrometry services, Creative Proteomics supports the qualitative and quantification of multiple types of chlorophyll, and has a good record. We can meet your specific project requirements from sample extraction and detection to bioinformatics. https://metabolomics.creat...

Creative Proteomics has established a carotenoids analysis method based on the LC-MS/MS platform external standard + isotope internal standard, which can detect a wide range of carotenoids. This method has low throughput, qualitative accuracy and can complete the absolute quantification of metabolites. Creative Proteomics can provide technical services for the entire process from sample extraction to presentation of results report. https://metabolomics.creat...

Within the intricate tapestry of plant biology, plant metabolites stand as the small but mighty actors, orchestrating a multitude of chemical reactions that underpin the life of plants. These metabolites encompass a broad spectrum of molecules, each with its unique role and significance. Among them, primary metabolites such as sugars, amino acids, organic acids, and nucleotides serve as the foundational building blocks for essential cellular processes. They are the currencies of energy production, structural formation, and growth regulation within plant cells. Plant Targeted Metabolomics focuses on quantifying specific plant metabolites of interest. This approach involves selecting a predefined set of metabolites and accurately measuring their concentrations. https://metabolomics.creat...

In addition, Creative Proteomics can provide a variety of exosome isolation and extraction solutions according to the characteristics of customer samples, such as filtration centrifugation, kit extraction, etc. Also, we provide customized exosome proteomics analysis to identify and quantify the proteins in the exosomes with the obtained high-purity exosome particles. https://www.creative-prote...

Up to now, various methods have been developed for quantifying cellular levels of dNTPs, including enzymatic and liquid chromatography assays. The latter involves direct separation of dNTPs by high-performance liquid chromatography (HPLC), which can be detected using UV or tandem mass spectrometry. Ideally, a combination of these two methods should be used for sensitive and reliable quantification. Similarly, the identification and quantification of cyclic-di-AMP would be more effectively achieved through the utilization of LC-MS/MS. With integrated set of separation, characterization, identification, and quantification systems featured with excellent robustness & reproducibility, high sensitivity, Creative Proteomics provides reliable, rapid, and cost-effective deoxynucleotide triphosphates (dNTPs) and cyclic-di-AMP targeted metabolomics services. https://www.creative-prote...

Peptide synthesis entails the process of pairing carboxyl groups (referred to as the C-terminus) and amino groups (referred to as the N-terminus). Due to the potential occurrence of unexpected reactions, protective groups become imperative. The chemical synthesis of peptides initiates from the C-terminus and concludes at the N-terminus, while protein biosynthesis follows the opposite direction, commencing at the N-terminus and terminating at the C-terminus. https://www.creative-prote...

RIP technology mainly uses antibodies against target proteins to precipitate the corresponding RNA-protein complexes, which can be separated and purified for q-PCR validation or sequencing analysis of the RNA bound to the complexes. RIP is a technique to study intracellular RNA-protein binding, and its application fields include post-transcriptional regulation studies and epigenetic regulation. RNA-binding proteins (RBPs) are involved in the transcription and maturation of mRNAs, including biological processes such as processing, translocation, stabilization, degradation and translation; similarly, some mRNA molecules are regulated by ribonucleoprotein complexes (RNPs) at the post-transcriptional level ; RIP is a powerful tool to understand the dynamic processes of post-transcriptional regulatory networks and can help discover the regulatory targets of miRNAs. https://www.iaanalysis.com...

Creative Proteomics has established a sophisticated molecular experimental platform. All you need to do is provide the target protein antibody (available for Western Blot) and its instructions, the cell sample to be tested, the target gene, and the target protein information. Our skilled technical team will then provide you with RNA Pull-Down Result figure, raw data, result figure (including Input, positive and negative control results), and standard experiment report (including detailed materials and methods). https://www.iaanalysis.com...

Ceramide has a strong ability to associate with water molecules. It maintains skin moisture by forming a network structure in the stratum corneum. In addition, it also plays an important role in maintaining the integrity of cell membranes, cell stress response, inflammatory signal transduction and cell apoptosis. Creative Proteomics uses liquid chromatography mass spectrometry (LC-MS) method to identify the samples, which can effectively and accurately detect the changes in the content of ceramide. https://lipidomics.creativ...

"Steroids function as important components of cell membranes and many of them are signaling molecules. All steroids are manufactured in cells from the sterols lanosterol (animals and fungi) or cycloartenol (plants). Lanosterol and cycloartenol are derived from the cyclization of the triterpene squalene.

With an integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective sterol lipids analysis services." https://lipidomics.creativ...

Our canine Luminex multiplex assay service gets the ideal speed and sensitivity advantage, allowing simultaneous analysis of factors and multiple cytokines, chemotactic other protein biomarkers, increasing the efficiency of researchers and allowing you to focus on the potential role of cytokines in disease, and the regulation of cytokine expression. https://cytokine.creative-...

Luminex multiplex detection is driven by xMAP technology, which can simultaneously detect and quantify more than 500 protein molecules, including cytokines, hormones, autoantibodies, tumor markers, etc. Creative Proteomics provides Luminex multiplex assay platform to help you accelerate the progress of the project, suitable for basic and clinical medical research. https://cytokine.creative-...

Creative Proteomics has multiple cytokine detection platforms such as has multiplex luminex, flow cytometry, and enzyme-linked immunosorbent technology. Each platform has its advantages. We will select the appropriate technology combination according to your project to meet your various needs and help researchers to obtain higher quality scientific research data. https://cytokine.creative-...

Mass spectrometry-based profiling of saponins enables quantitative analyses of saponins in a faster, convenient, and sensitive manner. With decades of experience in mass spectrometry services, Creative Proteomics has a proven track record supporting diverse saponins detection and quantification. We can meet your specific project requirements, from sampling to bioinformatics. https://metabolomics.creat...

The carbohydrate metabolism analysis has been applied in a wide range of fields including biomarker discovery, human disease research, clinical diagnostics, drug discovery, food safety, nutrition and systems biology. With integrated array of separation, characterization, identification and quantification experience, Creative Proteomics provides reliable, rapid and cost-effective carbohydrate metabolism services for your various scientific purposes. https://metabolomics.creat...